Correctly reconstituting peptides is imperative. Reconstituting peptides incorrectly can damage or even destroy the dell peptide bonds, rendering a given compound potentially inactive and thus useless. It is also critical to store peptides properly reduce degradation and damage.
Let’s talk about how and why to reconstitute peptides.
BACTERIOSTATIC WATER VS. STERILE WATER
Some people confuse bacteriostatic water with sterile water. For the purposes of this article, we only recommend using bacteriostatic water to reconstitute peptides.
Bacteriostatic water is a form of sterile water with small amounts of alcohol added to prevent bacterial growth. Reconstituting peptides properly helps minimize ar
eliminate damage to your active compound (the peptide itself).
HOW TO RECONSTITUTE PEPTIDES
Start by using an alcohol wipe to clean the top of your peptide vial Next, you’ll want to add enough bacteriostatic water to the peptide vial so that you end up with the proper concentration that you are targeting. Typical peptide vials will hold 2/2.5mL of bacteriostatic water at most. Make sure to also wipe down the bacteriostatic water before inserting the needle. You will likely want to use a larger syringe (i.e. a 3mL syringe) to add the bacteriostatic water to the peptide vial.
Let’s say, for an easy example, that you are adding 2mL of bacteriostatic water. After filling the 3mL syringe with the appropriate amount of bacteriostatic water (@ml.in this example), carefully insert the needle into the peptide vial. Some peptide vials have a vacuum (pressure) in the vial. This will cause the bacteriostatic water to shoot into the peptide vial rapidly. Be careful to avoid this. Do not let the water inject directly onto the lyophilized powder. This can damage the peptide, Angle the needle
toward the side of the peptide vial, and slowly inject it so it drips down and mixes with the lyophilized powder.
NOTE: whether there is a vacuum in the peptide vial or not, is NOT an indicator of product quality whatsoever.
DO NOT SHAKE VIAL to speed up mixing, Gently swirl the vial until the lyophilized power is fully reconstituted, and then store the peptide vial in the refingerator. You may not need to swirl the peptide vial, as high quality peptides will dissolve on their own in almost all cases.
Post time: May-28-2024